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Extracellular Expression of thermostable Geobacillus stearothermophilus T1.2RQ Lipase for Biodiesel Production / Elemosho Ridwan

Detail koleksi

Extracellular Expression of thermostable Geobacillus stearothermophilus T1.2RQ Lipase for Biodiesel Production / Elemosho Ridwan

Pengarang
Elemosho Ridwan Suwanto, Antonius (Pembimbing I) Thenawidjaja, Maggy (Pembimbing II)
Jenis bahan
Tesis
Edisi
-
Penerbitan
Bogor : IPB University, 2021
Deskripsi fisik
vi, 44 halaman : Ilustrasi ; 29 cm.
Bahasa
-
ISBN / ISSN / ISMN
-
Subjek
Sekolah Pascasarjana-- Bioteknologi Biotechnology-- Enzyme-- 2020-- Bekasi - Jawa Barat
Abstrak
The extracellular expression of enzymes in an excellent secretion host such as Bacillus subtilis is a useful strategy for making enzymes of industrial significance more affordable by lowering the cost of its downstream processing. Lipases are a category of hydrolases that catalyze the hydrolysis of triglycerides to glycerol and free fatty acids. Also, lipases catalyze the hydrolysis and transesterification of other esters as well as the synthesis of esters. They can perform very specific biotransformation reactions and are widely used in food, organic synthesis, detergents, cosmetic, and pharmaceutical industries. Geobacillus stearothermophilus T1.2RQ lipase is an industrially-desired thermostable lipolytic enzyme with an excellent hydrolytic and transesterification activity. This study aimed to express T1.2RQ lipase, in B. subtilis WB800 and ensure its secretion into the extracellular medium. Three signal peptides (AmyQ, Epr, LipA) and two promoters (Pgrac01 and Pgrac100) were used in the secretory-expressio
Catatan
Tesis (Magister).--IPB University, 2021
Akses online
https://repository.ipb.ac.id/handle/123456789/106649
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